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AQA GCSE Biology flashcards: the required practicals

All 10 required practicals for AQA GCSE Biology Triple Science: method marking points, key words, exam practice and examiner traps, written by an AQA Biology specialist.

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Required Practical 1: MicroscopyMarking points
Question
RP1: describe how to prepare a microscope slide.
Answer
Plant cells (for example onion): use forceps to peel off a thin layer of tissue and place it on a drop of water on a glass slide
Animal cells (for example cheek cells): swab the inside of the cheek with a cotton bud and smear the cells onto the centre of the slide
Then for both:
  • Add a drop of stain using a pipette, to make sub-cellular structures visible
  • Lower a coverslip at an angle onto the specimen to avoid trapping air bubbles
Tutor tip
The stain and the angled coverslip are the two steps students most often leave out.
Required Practical 1: MicroscopyMarking points
Question
RP1: describe how to use the microscope.
Answer
  • Clip the slide onto the stage and switch on the light, or adjust the mirror
  • Select the lowest power objective lens, usually x4
  • Use the coarse focusing dial to bring the stage close to the lens, then slowly turn it to move the stage away until the image comes into focus
  • Use the fine focusing dial to sharpen the image
  • Switch to a higher power objective lens and refocus using the fine dial only
Tutor tip
Focusing by moving the stage away from the lens stops you driving the lens into the slide and cracking it.
Required Practical 1: MicroscopyApplication
Question
RP1: how do you calculate total magnification?
Answer
Total magnification = magnification of eyepiece lens x magnification of objective lens
For example, a x10 eyepiece with a x4 objective gives x40 total magnification.

What is inside

50 cards covering 10 topics, each written to the AQA mark scheme. Cards are tagged as definitions, marking points, applications or examiner traps, and the bold words are the exact key words that earn the marks.

  • Required Practical 1: Microscopy
  • Required Practical 2: Microbiology (Biology only)
  • Required Practical 3: Osmosis
  • Required Practical 4: Food tests
  • Required Practical 5: Enzymes
  • Required Practical 6: Photosynthesis
  • Required Practical 7: Reaction time
  • Required Practical 8: Plant responses (Biology only)
  • Required Practical 9: Sampling
  • Required Practical 10: Decay (Biology only)

Who it is for

These flashcards are for AQA GCSE Biology (separate/Triple) students preparing for the practical questions that appear across both papers. They cover the exact methods, controls and calculations examiners test, from aseptic technique to sampling with a quadrat.

How access works

The first three cards are free to read on this page. To unlock the full deck, buy it once for £5.99. This is a one-time payment, not a subscription.

Access is then permanent and works on any device you sign in on, using the same account as Phlo AI. Payment is handled securely by Stripe.

Frequently asked questions

Is this deck for the AQA specification?

Yes. It is written for AQA GCSE Biology (8461), the separate/Triple Science course, and covers all 10 required practicals. It is not affiliated with AQA.

Does it cover all 10 required practicals?

Yes. Microscopy, microbiology, osmosis, food tests, enzymes, photosynthesis, reaction time, plant responses, sampling and decay. There are 50 cards, and the two microbiology and decay practicals are Biology-only, as flagged on the cards.

Is this for Combined Science or Triple?

This deck is written for the separate/Triple Biology course, which has all 10 required practicals. Combined Science students do a subset, so some cards (the Biology-only practicals) go beyond Combined.

Do I get lifetime access?

Yes. It is a one-time payment, not a subscription. Once you buy the deck you keep access permanently on any device you sign in on.

Can I try it before I buy?

Yes. The first three cards are free to read on this page. Buy once to unlock all 50.

How is this different from a textbook?

Every bold word is an exact mark-scheme key word that earns a mark, and the notes flag the examiner traps that lose them. It is written to be revised, not read.

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